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rabbit slit3 pab  (Bioss)


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    Structured Review

    Bioss rabbit slit3 pab
    The <t>SLIT3/ROBO1</t> axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm
    Rabbit Slit3 Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+slit3+pab/pmc13081268-82-25-30?v=Bioss
    Average 93 stars, based on 11 article reviews
    rabbit slit3 pab - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells"

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-026-04960-3

    The SLIT3/ROBO1 axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm
    Figure Legend Snippet: The SLIT3/ROBO1 axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm

    Techniques Used: Expressing, Co-Culture Assay, Staining

    Effects of shRNA lentivirus and neutralizing antibodies on SLIT3 and ROBO1 expression in EPCs/PBMSCs sheets. A, B The effects of shRNA lentivirus on SLIT3 protein and mRNA expression in PBMSCs were detected by RT-qPCR ( A ) and western blotting assays ( B ) ( N = 3). C The effect of SLIT3 shRNA lentivirus and ROBO1-neutralizing antibodies on SLIT3 levels in the supernatant of EPCs/PBMSCs sheets was detected by ELISA assay ( N = 3). D Representative images of gel bot for SLIT3 and ROBO1 in EPCs/PBMSCs sheets ( N = 3). Original gel blots see supplementary material
    Figure Legend Snippet: Effects of shRNA lentivirus and neutralizing antibodies on SLIT3 and ROBO1 expression in EPCs/PBMSCs sheets. A, B The effects of shRNA lentivirus on SLIT3 protein and mRNA expression in PBMSCs were detected by RT-qPCR ( A ) and western blotting assays ( B ) ( N = 3). C The effect of SLIT3 shRNA lentivirus and ROBO1-neutralizing antibodies on SLIT3 levels in the supernatant of EPCs/PBMSCs sheets was detected by ELISA assay ( N = 3). D Representative images of gel bot for SLIT3 and ROBO1 in EPCs/PBMSCs sheets ( N = 3). Original gel blots see supplementary material

    Techniques Used: shRNA, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    Effect of the SLIT3/ROBO1 axis on angiogenic–osteogenic coupling in EPCs/PBMSCs sheets. A Representative pictures of alizarin red staining for each group of EPCs/PBMSCs sheets ( N = 3). B Representative images of gel bolt for osteogenesis-related markers (ALP, RUNX2, and OCN) and vascular-related markers (CD31) ( N = 3). C The effects of the SLIT3/ROBO1 axis on vascular-osteogenic coupling of EPCs/PBMSCs sheets were observed by IF staining ( N = 3). Scale: 100 μm. The co-localization of CD31 and EMCN was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Original gel blots see supplementary material
    Figure Legend Snippet: Effect of the SLIT3/ROBO1 axis on angiogenic–osteogenic coupling in EPCs/PBMSCs sheets. A Representative pictures of alizarin red staining for each group of EPCs/PBMSCs sheets ( N = 3). B Representative images of gel bolt for osteogenesis-related markers (ALP, RUNX2, and OCN) and vascular-related markers (CD31) ( N = 3). C The effects of the SLIT3/ROBO1 axis on vascular-osteogenic coupling of EPCs/PBMSCs sheets were observed by IF staining ( N = 3). Scale: 100 μm. The co-localization of CD31 and EMCN was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Original gel blots see supplementary material

    Techniques Used: Staining

    Effect of the SLIT3/ROBO1 axis on bone regeneration mediated by EPCs/PBMSCs in alveolar bone defect models was detected by Micro CT. A Representative images of Micro CT of alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). B Differential expression analysis of BV/TV, Tb.Th, Tb.N and Tb.Sp of regenerated alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3)
    Figure Legend Snippet: Effect of the SLIT3/ROBO1 axis on bone regeneration mediated by EPCs/PBMSCs in alveolar bone defect models was detected by Micro CT. A Representative images of Micro CT of alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). B Differential expression analysis of BV/TV, Tb.Th, Tb.N and Tb.Sp of regenerated alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3)

    Techniques Used: Micro-CT, Quantitative Proteomics

    Effect of the SLIT3/ROBO1 axis on the therapeutic efficacy of EPCs/PBMSCs was characterized in alveolar bone defect models using tissue staining. A, B Bone regeneration of alveolar bone defect models in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups was evaluated by HE ( A ) and MASSON ( B ) staining ( N = 3). Scale: 3–500 μm. C Representative images of IHC staining for SLIT3, ROBO1, CD31 and EMCN proteins in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). Scale: 3–500 μm
    Figure Legend Snippet: Effect of the SLIT3/ROBO1 axis on the therapeutic efficacy of EPCs/PBMSCs was characterized in alveolar bone defect models using tissue staining. A, B Bone regeneration of alveolar bone defect models in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups was evaluated by HE ( A ) and MASSON ( B ) staining ( N = 3). Scale: 3–500 μm. C Representative images of IHC staining for SLIT3, ROBO1, CD31 and EMCN proteins in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). Scale: 3–500 μm

    Techniques Used: Drug discovery, Staining, Immunohistochemistry



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    Bioss rabbit slit3 pab
    The <t>SLIT3/ROBO1</t> axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm
    Rabbit Slit3 Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+slit3+pab/pmc13081268-82-25-30?v=Bioss
    Average 93 stars, based on 1 article reviews
    rabbit slit3 pab - by Bioz Stars, 2026-07
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    		  Buy from Supplier

    Image Search Results


    The SLIT3/ROBO1 axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm

    Journal: Stem Cell Research & Therapy

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    doi: 10.1186/s13287-026-04960-3

    Figure Lengend Snippet: The SLIT3/ROBO1 axis expression in EPCs/PBMSCs sheets. A Representative pictures of EPCs/PBMSCs sheets at 1 d, 3 d, 7 d, 10 d, and 14 d of co-culture. The PBMSCs and EPCs were respectively circled with blue and orange dotted lines. Scale: 20–500 μm. B The expression and localization of the SLIT3/ROBO1 axis in EPCs/PBMSCs sheets were detected by IF staining and statistically analyzed ( N = 3). The co-localization of SLIT3 and ROBO1 was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Scale: 500 μm

    Article Snippet: The closed alveolar bone sections were successively incubated with corresponding antibodies, including Mouse CD31 mAb (1:80; MA1-80069, Thermo Scientific), Rabbit EMCN-Cy5 pAb (1:100; bs-5884R, Bioss), Rabbit SLIT3 pAb (1:100; bs-7880R, Bioss), and Rabbit ROBO1-Cy5 pAb (1:150; bs-5794R, Bioss).

    Techniques: Expressing, Co-Culture Assay, Staining

    Effects of shRNA lentivirus and neutralizing antibodies on SLIT3 and ROBO1 expression in EPCs/PBMSCs sheets. A, B The effects of shRNA lentivirus on SLIT3 protein and mRNA expression in PBMSCs were detected by RT-qPCR ( A ) and western blotting assays ( B ) ( N = 3). C The effect of SLIT3 shRNA lentivirus and ROBO1-neutralizing antibodies on SLIT3 levels in the supernatant of EPCs/PBMSCs sheets was detected by ELISA assay ( N = 3). D Representative images of gel bot for SLIT3 and ROBO1 in EPCs/PBMSCs sheets ( N = 3). Original gel blots see supplementary material

    Journal: Stem Cell Research & Therapy

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    doi: 10.1186/s13287-026-04960-3

    Figure Lengend Snippet: Effects of shRNA lentivirus and neutralizing antibodies on SLIT3 and ROBO1 expression in EPCs/PBMSCs sheets. A, B The effects of shRNA lentivirus on SLIT3 protein and mRNA expression in PBMSCs were detected by RT-qPCR ( A ) and western blotting assays ( B ) ( N = 3). C The effect of SLIT3 shRNA lentivirus and ROBO1-neutralizing antibodies on SLIT3 levels in the supernatant of EPCs/PBMSCs sheets was detected by ELISA assay ( N = 3). D Representative images of gel bot for SLIT3 and ROBO1 in EPCs/PBMSCs sheets ( N = 3). Original gel blots see supplementary material

    Article Snippet: The closed alveolar bone sections were successively incubated with corresponding antibodies, including Mouse CD31 mAb (1:80; MA1-80069, Thermo Scientific), Rabbit EMCN-Cy5 pAb (1:100; bs-5884R, Bioss), Rabbit SLIT3 pAb (1:100; bs-7880R, Bioss), and Rabbit ROBO1-Cy5 pAb (1:150; bs-5794R, Bioss).

    Techniques: shRNA, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    Effect of the SLIT3/ROBO1 axis on angiogenic–osteogenic coupling in EPCs/PBMSCs sheets. A Representative pictures of alizarin red staining for each group of EPCs/PBMSCs sheets ( N = 3). B Representative images of gel bolt for osteogenesis-related markers (ALP, RUNX2, and OCN) and vascular-related markers (CD31) ( N = 3). C The effects of the SLIT3/ROBO1 axis on vascular-osteogenic coupling of EPCs/PBMSCs sheets were observed by IF staining ( N = 3). Scale: 100 μm. The co-localization of CD31 and EMCN was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Original gel blots see supplementary material

    Journal: Stem Cell Research & Therapy

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    doi: 10.1186/s13287-026-04960-3

    Figure Lengend Snippet: Effect of the SLIT3/ROBO1 axis on angiogenic–osteogenic coupling in EPCs/PBMSCs sheets. A Representative pictures of alizarin red staining for each group of EPCs/PBMSCs sheets ( N = 3). B Representative images of gel bolt for osteogenesis-related markers (ALP, RUNX2, and OCN) and vascular-related markers (CD31) ( N = 3). C The effects of the SLIT3/ROBO1 axis on vascular-osteogenic coupling of EPCs/PBMSCs sheets were observed by IF staining ( N = 3). Scale: 100 μm. The co-localization of CD31 and EMCN was analyzed using Image J and the Coloc 2 plugin, characterized by the Person’s Coefficient and the Overlap Coefficient. Original gel blots see supplementary material

    Article Snippet: The closed alveolar bone sections were successively incubated with corresponding antibodies, including Mouse CD31 mAb (1:80; MA1-80069, Thermo Scientific), Rabbit EMCN-Cy5 pAb (1:100; bs-5884R, Bioss), Rabbit SLIT3 pAb (1:100; bs-7880R, Bioss), and Rabbit ROBO1-Cy5 pAb (1:150; bs-5794R, Bioss).

    Techniques: Staining

    Effect of the SLIT3/ROBO1 axis on bone regeneration mediated by EPCs/PBMSCs in alveolar bone defect models was detected by Micro CT. A Representative images of Micro CT of alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). B Differential expression analysis of BV/TV, Tb.Th, Tb.N and Tb.Sp of regenerated alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3)

    Journal: Stem Cell Research & Therapy

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    doi: 10.1186/s13287-026-04960-3

    Figure Lengend Snippet: Effect of the SLIT3/ROBO1 axis on bone regeneration mediated by EPCs/PBMSCs in alveolar bone defect models was detected by Micro CT. A Representative images of Micro CT of alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). B Differential expression analysis of BV/TV, Tb.Th, Tb.N and Tb.Sp of regenerated alveolar bone in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3)

    Article Snippet: The closed alveolar bone sections were successively incubated with corresponding antibodies, including Mouse CD31 mAb (1:80; MA1-80069, Thermo Scientific), Rabbit EMCN-Cy5 pAb (1:100; bs-5884R, Bioss), Rabbit SLIT3 pAb (1:100; bs-7880R, Bioss), and Rabbit ROBO1-Cy5 pAb (1:150; bs-5794R, Bioss).

    Techniques: Micro-CT, Quantitative Proteomics

    Effect of the SLIT3/ROBO1 axis on the therapeutic efficacy of EPCs/PBMSCs was characterized in alveolar bone defect models using tissue staining. A, B Bone regeneration of alveolar bone defect models in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups was evaluated by HE ( A ) and MASSON ( B ) staining ( N = 3). Scale: 3–500 μm. C Representative images of IHC staining for SLIT3, ROBO1, CD31 and EMCN proteins in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). Scale: 3–500 μm

    Journal: Stem Cell Research & Therapy

    Article Title: SLIT3/ROBO1 axis contributes to angiogenic–osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells

    doi: 10.1186/s13287-026-04960-3

    Figure Lengend Snippet: Effect of the SLIT3/ROBO1 axis on the therapeutic efficacy of EPCs/PBMSCs was characterized in alveolar bone defect models using tissue staining. A, B Bone regeneration of alveolar bone defect models in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups was evaluated by HE ( A ) and MASSON ( B ) staining ( N = 3). Scale: 3–500 μm. C Representative images of IHC staining for SLIT3, ROBO1, CD31 and EMCN proteins in the EPCs/PBMSCs, EPCs/shNC-PBMSCs, EPCs/shSLIT3-PBMSCs, anti-ROBO1-EPCs/PBMSCs, and anti-ROBO1-EPCs/shSLIT3-PBMSCs groups ( N = 3). Scale: 3–500 μm

    Article Snippet: The closed alveolar bone sections were successively incubated with corresponding antibodies, including Mouse CD31 mAb (1:80; MA1-80069, Thermo Scientific), Rabbit EMCN-Cy5 pAb (1:100; bs-5884R, Bioss), Rabbit SLIT3 pAb (1:100; bs-7880R, Bioss), and Rabbit ROBO1-Cy5 pAb (1:150; bs-5794R, Bioss).

    Techniques: Drug discovery, Staining, Immunohistochemistry